Plasmid_Backbone

Part:BBa_K3728010:Design

Designed by: Eric Shih   Group: iGEM21_Mingdao   (2021-08-23)


pSB6C1 - a pBR322-based vector with Chloramphenicol resistance


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3819
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3825
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3819
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3819
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3819
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3834
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 43
    Illegal NgoMIV site found at 411
    Illegal NgoMIV site found at 571
    Illegal NgoMIV site found at 925
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 1992


Design Notes

N/A


Source

Exchange AmpR of pSB6A1 with KanR

References